Differential role for competitive reverse transcriptase-polymerase chain reaction and intracellular cytokine staining as diagnostic tools for the assessment of intragraft cytokine profiles in rejecting and nonrejecting heart allografts.

Journal article

The early and reliable diagnosis of allograft rejection is a difficult task and the assessment of cytokine expression in the grafts can be a helpful parameter. We have compared competitive reverse transcriptase-polymerase chain reaction (RT-PCR) with intracellular cytokine staining by flow cytometry as tools to measure cytokine expression in rejecting and nonrejecting murine cardiac allografts. Both techniques gave comparable results for cytokine expression in rejecting allografts and syngene...

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Authors: Spriewald, B; Hara, M; Bushell, A; Jenkins, S; Morris, P

• Publication status: Published
• Journal: American journal of pathology
• Volume: 157
• Issue: 5
• Pages: 1453-1458
• Publication date: 2000-11-01
• DOI: 10.1016/s0002-9440(10)64783-9
• EISSN: 1525-2191
• ISSN: 0002-9440
• Language: English
• Keywords: RNA, Messenger; Intracellular Membranes; Staining and Labeling; Heart Transplantation; Up-Regulation; Tetradecanoylphorbol Acetate; Heart; Graft Rejection; Ionomycin; Mice, Inbred C57BL; Ionophores; Transplantation, Homologous; Animals; Mice, Inbred CBA; Reverse Transcriptase Polymerase Chain Reaction; Myocardium; Cytokines; Mice