The emerging role of MS in structure elucidation of protein-nucleic acid complexes.

Journal article

Developments in MS enable us to apply this technique to non-covalent complexes, defining their stoichiometry, subunit interactions and architectural organization. We illustrate the application of this non-covalent MS approach to uncovering the overall topological arrangements of subunits and interactions within RNA-protein complexes studied in our laboratory over the last 5 years. These studies exemplify the emerging role and potential of MS as a complementary structural biology methodology and demonstrate its unique niche in investigations of dynamic or heterogeneous protein-nucleic acid complexes, which are not accessible to classical high-resolution structural biology techniques.

Authors: Gordiyenko, Y; Robinson, C

• Publication status: Published
• Journal: Biochemical Society transactions
• Volume: 36
• Issue: Pt 4
• Pages: 723-731
• Publication date: 2008-08-01
• DOI: 10.1042/bst0360723
• EISSN: 1470-8752
• ISSN: 0300-5127
• Language: English
• Keywords: Nucleic Acids; Mass Spectrometry; Humans; Proteins; Protein Binding; Gene Expression