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Serial cryoFIB/SEM reveals cytoarchitectural disruptions in Leigh syndrome patient cells

Abstract:

The advancement of serial cryoFIB/SEM offers an opportunity to study large volumes of near-native, fully hydrated frozen cells and tissues at voxel sizes of 10 nm and below. We explored this capability for pathologic characterization of vitrified human patient cells by developing and optimizing a serial cryoFIB/SEM volume imaging workflow. We demonstrate profound disruption of subcellular architecture in primary fibroblasts from a Leigh syndrome patient harboring a disease-causing mutati...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1016/j.str.2020.10.003

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Role:
Author
ORCID:
0000-0003-0220-8844
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Publisher:
Cell Press Publisher's website
Journal:
Structure Journal website
Volume:
29
Issue:
1
Pages:
82-87.e3
Publication date:
2020-10-22
Acceptance date:
2020-10-05
DOI:
EISSN:
1878-4186
ISSN:
0969-2126
Pmid:
33096015
Language:
English
Keywords:
Pubs id:
1139635
Local pid:
pubs:1139635
Deposit date:
2021-07-14

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