Journal article
Serial cryoFIB/SEM reveals cytoarchitectural disruptions in Leigh syndrome patient cells
- Abstract:
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The advancement of serial cryoFIB/SEM offers an opportunity to study large volumes of near-native, fully hydrated frozen cells and tissues at voxel sizes of 10 nm and below. We explored this capability for pathologic characterization of vitrified human patient cells by developing and optimizing a serial cryoFIB/SEM volume imaging workflow. We demonstrate profound disruption of subcellular architecture in primary fibroblasts from a Leigh syndrome patient harboring a disease-causing mutati...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Version of record, 5.2MB)
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- Publisher copy:
- 10.1016/j.str.2020.10.003
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Authors
Funding
Wellcome Trust
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Bibliographic Details
- Publisher:
- Cell Press Publisher's website
- Journal:
- Structure Journal website
- Volume:
- 29
- Issue:
- 1
- Pages:
- 82-87.e3
- Publication date:
- 2020-10-22
- Acceptance date:
- 2020-10-05
- DOI:
- EISSN:
-
1878-4186
- ISSN:
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0969-2126
- Pmid:
-
33096015
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
1139635
- Local pid:
- pubs:1139635
- Deposit date:
- 2021-07-14
Terms of use
- Copyright holder:
- Zhu et al.
- Copyright date:
- 2021
- Rights statement:
- Copyright 2020 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
- Licence:
- CC Attribution (CC BY)
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