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Exploring PAZ/3′-overhang interaction to improve siRNA specificity: a combined experimental and modeling study

Abstract:

The understanding of the dynamical and mechanistic aspects that lie behind siRNA-based gene regulation is a requisite to boost the performance of siRNA therapeutics. A systematic experimental and computational study on the 3′-overhang structural requirements for the design of more specific and potent siRNA molecules was carried out using nucleotide analogues differing in structural parameters, such as sugar constraint, lack of nucleobase, distance between the phosphodiester backbone and nucle...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1039/c8sc00010g

Authors


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Institution:
University of Oxford
Division:
MSD
Department:
Pathology Dunn School
Role:
Author
ORCID:
0000-0001-6421-7733
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Role:
Author
ORCID:
0000-0002-9415-7337
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Role:
Author
ORCID:
0000-0003-3047-738X
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Role:
Author
ORCID:
0000-0002-2840-6091
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Role:
Author
ORCID:
0000-0002-6687-382X
Publisher:
Royal Society of Chemistry
Journal:
Chemical Science More from this journal
Volume:
9
Issue:
8
Pages:
2074-2086
Publication date:
2018-01-15
Acceptance date:
2018-01-15
DOI:
EISSN:
2041-6539
ISSN:
2041-6520
Pmid:
29719684
Language:
English
Pubs id:
pubs:830351
UUID:
uuid:0088e204-5f00-4530-95ce-f8f872624b3d
Local pid:
pubs:830351
Source identifiers:
830351
Deposit date:
2018-06-07

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