Structural and biophysical studies of RNA-dependent RNA polymerases

Thesis

RNA-dependent RNA polymerases (RdRps) play a vital role in the life cycle of RNA viruses, being responsible for genome replication and mRNA transcription. In this thesis viral RdRps (vRdRps) of dsRNA bacteriophage phi6 (phi6 RdRp) and Severe Acute Respiratory Syndrome (SARS) coronavirus [non structural protein 12 (NSP-12)] are studied. For SARS polymerase NSP-12, a library-based screening method known as ESPRIT (Expression of Soluble Protein by Random Incremental Truncation) was employed in...

Expand abstract

Authors: Wright, S

• Publication date: 2010
• Type of award: DPhil
• Level of award: Doctoral
• Awarding institution: Oxford University, UK
• Language: English
• Keywords: RdRP; virus replication; SARS; RNA polymerase; bacteriophage phi6
• Subjects: Clinical microbiology; Microbiology; Life Sciences; Molecular biophysics (biochemistry); Biochemistry; Viruses; Structural genomics; Medical Sciences