Secreted frizzled-related protein-1 inhibits RANKL-dependent osteoclast formation.

Journal article

UNLABELLED: We determined that sFRP-1 mRNA was differentially expressed by osteoblast/stromal cell lines and that sFRP-1 neutralizing antibodies and siRNA complementary to sFRP-1 coding sequence enhanced, while recombinant sFRP-1 inhibited, osteoclast formation. In studying the mechanism of action for sFRP-1, we found that sFRP-1 could bind recombinant RANKL. These results suggest potential cross-talk between Wnt and RANKL pathways. INTRODUCTION: Osteoclast formation in normal bone remodelin...

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Authors: Häusler, K; Horwood, N; Chuman, Y; Fisher, J; Ellis, J

• Publication status: Published
• Journal: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
• Volume: 19
• Issue: 11
• Pages: 1873-1881
• Publication date: 2004-11-01
• DOI: 10.1359/jbmr.040807
• EISSN: 1523-4681
• ISSN: 0884-0431
• Language: English
• Keywords: Wnt Proteins; Osteoclasts; RNA, Small Interfering; Male; Receptor Activator of Nuclear Factor-kappa B; Membrane Glycoproteins; Interleukin-6; Enzyme-Linked Immunosorbent Assay; In Situ Hybridization; Calcitriol; Escherichia coli; Osteoblasts; Recombinant Fusion Proteins; Mice, Inbred C57BL; Parathyroid Hormone; Bone Marrow Cells; Macrophage Colony-Stimulating Factor; Bone and Bones; RANK Ligand; Animals; Cell Line; RNA, Messenger; Dose-Response Relationship, Drug; Polymerase Chain Reaction; Carrier Proteins; Gene Expression Regulation; Recombinant Proteins; Chondrocytes; Intercellular Signaling Peptides and Proteins; Interleukin-11; Gene Expression Profiling; Glutathione Transferase; Signal Transduction; Membrane Proteins; Mice