Inferring structural properties of protein-DNA binding using high-throughput sequencing. The paradigm of GATA1, KLF1 and their complexes GATA1/FOG1 and GATA1/KLF1. Insights into the transcriptional regulation of the erythroid cell lineage.

Thesis

GATA1 and KLF1 are transcription factors that regulate genes which are important for the development of erythroid cells. The GATA1 transcriptional co-factor FOG1 has been shown to be essential in a wide range of GATA1 dependent cellular functions. Here we tried to understand the diverse mechanisms by which GATA1 and KLF1 recognize their binding sites, how the GATA1 recognition mechanisms are affected by complexation with either FOG1 or KLF1 and how the GATA1 recognition mechanisms affect t...

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Authors: Oikonomopoulos, S

• Publication date: 2014
• Type of award: DPhil
• Level of award: Doctoral
• Awarding institution: Oxford University, UK
• Language: English
• Keywords: gene regulation; genomics; High-throughput sequencing; EMSA-seq; GATA1; FOG1; KLF1
• Subjects: Medical Sciences; Computational biochemistry; Biology (medical sciences); Haematology; Molecular biophysics (biochemistry); Biology; Gene medicine; Genetics (medical sciences); Biochemistry; Molecular genetics; Genetics (life sciences); Molecular haematology; Bioinformatics (biochemistry); Structural genomics; Bioinformatics (life sciences)