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3D histology reconstruction with external blockface reference. Full mouse heart, 600 sections

Alternative title:
3D reconstruction of Picro sirius red histology data using Transformation Diffusion Reconstruction
Documentation:
Mouse heart Picro sirius red histology was reconstructed using the Transformation Diffusion Reconstruction (TDR) method in (Casero et al. 2017).

Histology slices were downsampled by a factor of x24.6 down to blockface pixel size, and pre-aligned to the blockface using rigid registration, followed by rigid TDR refinement (M=150 stack sweeps) and B-spline ATDR refinement (M=80). We then applied the low resolution refinement to the original histology downsampled only by a factor of 2. In this high-resolution stack we applied further B-spline ATDR refinement (M=10). R. Casero, U. Siedlecka, M. Gibb, J.E. Schneider, P. Kohl, V. Grau. "Transformation Diffusion Reconstruction of Three-Dimensional Histology Volumes from Two-Dimensional Image Stacks". Medical Image Analysis, 2017 This is the reconstruction presented in the (Casero et al. 2017) paper that introduced the Transformation Diffusion Reconstruction method. R. Casero, U. Siedlecka, M. Gibb, J.E. Schneider, P. Kohl, V. Grau. "Transformation Diffusion Reconstruction of Three-Dimensional Histology Volumes from Two-Dimensional Image Stacks". Medical Image Analysis, 2017.
Version:
1.0

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Authors/Creators

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Institution:
University of Oxford
Division:
MPLS
Department:
Engineering Science
Sub department:
Institute of Biomedical Engineering
Role:
Researcher, Creator
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Department:
Heart Science Centre, National Lung and Heart Institute, Imperial College London, Harefield UB9 6JH, UK.
Role:
Creator
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Department:
Heart Science Centre, National Lung and Heart Institute, Imperial College London, Harefield UB9 6JH, UK
Role:
Creator
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Department:
Heart Science Centre, National Lung and Heart Institute, Imperial College London, Harefield UB9 6JH, UK
Role:
Creator
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Department:
Department of Physiology and Biophysics, Dalhousie University, Halifax, NS B3H 4R2, Canada
Role:
Creator

Contributors

Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Human Genetics Wt Centre
Role:
Principal Investigator (PI)
Institution:
University of Oxford
Division:
MPLS
Department:
Engineering Science
Sub department:
Institute of Biomedical Engineering
Role:
Principal Investigator (PI)


More from this funder
Funder identifier:
https://ror.org/00k4n6c32
Funding agency for:
Casero, R
Grant:
FP7, 2008-2011
More from this funder
Funder identifier:
https://ror.org/02wdwnk04
Grant:
NH/13/30238
FS/11/50/29038
FS/12/17/29532
Funding agency for:
Schneider, J
Kohl, P
More from this funder
Funder identifier:
https://ror.org/0472cxd90
Funding agency for:
Kohl, P
More from this funder
Funder identifier:
https://ror.org/00cwqg982
Grant:
BB/I012117/1


Publisher:
Institute for Experimental Cardiovascular Medicine, University of Freiburg; Institute of Biomedical Engineering, Department of Engineering Science and Wellcome Trust Centre for Human Genetics, University of Oxford; Heart Science Centre, National Lung and Heart Institute, Imperial College London
Publication date:
2017
Version number:
1.0
DOI:


Language:
English
Keywords:
Subjects:
Pubs id:
2080973
UUID:
uuid:716fe2ef-f965-40ff-8da4-8a5ad48f4aea
Local pid:
pubs:2080973
Deposit date:
2017-03-28
ARK identifier:

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