ORA Thesis: "Investigating the regulation and functioning of RNT-1 and BRO-1 in C. elegans" - uuid:6d9cd263-1051-443a-b064-c0c329252b28



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Reference: Charles Brabin, (2012). Investigating the regulation and functioning of RNT-1 and BRO-1 in C. elegans. DPhil. University of Oxford.

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Title: Investigating the regulation and functioning of RNT-1 and BRO-1 in C. elegans


The stem cell-like seam cells of the nematode, Caenorhabditis elegans, represent a tractable and powerful model for studying stem cell biology. rnt-1, the worm homologue of the mammalian RUNX family of transcription factors, together with the CBFβ homologue bro-1, is essential for the proliferation of the seam cells. RUNX genes and CBFβ are important regulators of stem cell development in mammals, and are associated with a variety of cancers. The worm seam cell model offers an opportunity to examine how these genes function in stem cell biology. The aim of this work was to shed light on the genetic network in which bro-1 and rnt-1 function, and to reveal the identity of regulators of these genes as well the downstream targets of the bro-1/rnt-1 pathway.

Here, a number of genes that interact with bro-1 and rnt-1 have been identified. ELT-1, a GATA transcription factor, is shown to be a direct regulator of bro-1. Findings which show that the MEIS gene unc-62 acts upstream of bro-1/rnt-1 and regulates the symmetry of seam cell divisions are also presented. The seam cell marker, scm::gfp, is widely used in studies of the seam cells; here the results of an investigation into its identity and functional links are described. In addition, the mechanism underlying spatial regulation of rnt-1 was examined; this led to the discovery of distinct tissue-specific enhancer modules within an intron of this gene. Finally, interactions between pal-1 and bro-1/rnt-1 are reported and described.

Together, these findings provide a framework for furthering our understanding of the mechanisms and genes associated with the functioning of bro-1 and rnt-1 in the worm.

Digital Origin:Born digital
Type of Award:DPhil
Level of Award:Doctoral
Awarding Institution: University of Oxford
Notes:This thesis is not currently available via ORA.
About The Authors
institutionUniversity of Oxford
facultyMedical Sciences Division - Biochemistry
researchGroupWoollard laboratory
oxfordCollegeMagdalen College
Dr Alison Woollard More by this contributor
Bibliographic Details
Issue Date: 2012
Copyright Date: 2011
Urn: uuid:6d9cd263-1051-443a-b064-c0c329252b28
Item Description
Member of collection : ora:thesis
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Copyright Holder: Charles Brabin
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