Engineering a 2D protein-DNA crystal.

Journal article

(Figure Presented) Weaving with DNA: A DNA-binding protein was used to control the structure of a self-assembled 2D crystal. In the absence of protein, four oligonucleotides hybridize to form a Kagome lattice of interwoven double helices with p3 symmetry (see image). Addition of protein RuvA during assembly changes the symmetry and connectivity to give a DNA-protein crystal with an approximately square unit cell. © 2005 Wiley-VCH Verlag GmbH and Co. KGaA.

Authors: Malo, J; Mitchell, J; Vénien-Bryan, C; Harris, JR; Wille, H

• Publication status: Published
• Journal: Angewandte Chemie (International ed. in English)
• Volume: 44
• Issue: 20
• Pages: 3057-3061
• Publication date: 2005-05-01
• DOI: 10.1002/anie.200463027
• EISSN: 1521-3773
• ISSN: 1433-7851
• Language: English
• Keywords: Escherichia coli Proteins; Oligonucleotide Array Sequence Analysis; Crystallization; Protein Binding; DNA Helicases; Nucleic Acid Hybridization; Base Sequence; DNA-Binding Proteins; DNA, Cruciform; Nanotechnology; DNA; Molecular Sequence Data; Microscopy, Electron