Analysis of mouse models of insulin secretion disorders

Thesis

In this thesis, three mouse models and cell culture techniques were used to investigate genetic factors leading to glucose intolerance, diabetes or hyperinsulinemia.

1. Loss of nicotinamide nucleotide transhydrogenase (Nnt) function is linked to ROS-mediated uncoupling of mitochondrial metabolism and reduced insulin secretion. The enzymatic activity of Nnt generates mitochondrial NADPH essential for ROS detoxification. However, the exact nature of ROS as well as the ant...

Expand abstract

Authors: Kaizik, S

• Publication date: 2010
• DOI: 10.5287/ora-zbrpnnqgx
• Type of award: DPhil
• Level of award: Doctoral
• Awarding institution: University of Oxford
• Language: English
• Keywords: NADP; Potassium Channels; Menadione; Glibenclamide; Calcium Oscillations; ROS Measurement; Tolbutamide; Hyperinsulinemia; Calcium Imaging; Electrophysiology; Inwardly Rectifying; Quantitative PCR; Calcium Signaling; Pancreatic Islet Isolation; Reactive Oxygen Species; SUR1; Disease Models; RNA Interference; Transgenic Mice; Insulin Secretion; Sulfonylurea; Glutathione Peroxidase; Western Blotting; Neonatal diabetes; Nicotinamide Nucleotide Transhydrogenase; GSH; V59M; SUR1-E1506K; Genetic Background; Adenosine Triphosphate; KATP channels; Islets of Langerhans; Glutathione; NNT
• Subjects: Medical Sciences; Microscopy; Life Sciences; Genetics (medical sciences); Biochemistry; Pharmacology; Metabolism; Molecular Biology; Transgenics; Genetics (life sciences); Biology; Endocrinology; Physiology; Diabetes